Scorecard
Lee claimed five for 49 from nine overs to restrict England to just 220 all out from 46.3 overs after they had won the toss in good batting conditions on a sunny morning. It was the ninth time Lee had taken five wickets in a One-Day International.
Captain Andrew Strauss continued his good form over the English summer with a top score of 63 in 75 deliveries but he was the only England player to craft any substantial innings.
Australia reached 221/3 in reply with 6.2 overs remaining.
Michael Clarke, who stood in as captain in the first three matches when Ricky Ponting took a break in Australia, finished the match with an unbeaten 62, while opener Tim Paine made 51 and Ponting stroked 48.
While Australia will be seeking a 7-0 whitewash after losing the Ashes series 2-1, England need to regain credibility and confidence before the ICC Champions Trophy begins in South Africa later this month.
Lee, who misses the entire Ashes series through injury, made the initial breakthrough when he had Joe Denly caught at first slip for 11. He later bowled Matt Prior (29), Luke Wright (12), Adil Rashid (4) and Stuart Broad (2) with dipping, swinging yorkers in excess of 145 kms an hour.
England lost their last five wickets for just 20 runs in 22 balls. Off-spinner Nathan Hauritz claimed two for 23 from 10 accurate overs that strangled the England run rate.
Strauss was out top-edging a slog-sweep off Hauritz, while Owais Shah was caught for 39 from a Shane Watson full toss that he felt should have been called a no-ball
